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cloning vector a1  (Addgene inc)


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    Structured Review

    Addgene inc cloning vector a1
    Cloning Vector A1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloning+vector+a1/pAc5%2E1B-lambdaN-HA-DmMe31B+(Plasmid+%2321683)/us11530424-153-13-64
    Average 93 stars, based on 4 article reviews
    cloning vector a1 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Efficient CRISPR/HDR-mediated knock-in system and method of use
    Article Snippet: .. The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651-12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Article Title: CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.
    Article Snippet: .. 58 | No. 4 | 2015 and the resulting clone was named pX330-LAsg. b) PCR amplification of DNA templates for synthesis of sgRNAs by in vitro transcription with T7 RNA polymerase: The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651–12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Cloning:

    Article Title: Efficient CRISPR/HDR-mediated knock-in system and method of use
    Article Snippet: .. The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651-12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Article Title: CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.
    Article Snippet: .. 58 | No. 4 | 2015 and the resulting clone was named pX330-LAsg. b) PCR amplification of DNA templates for synthesis of sgRNAs by in vitro transcription with T7 RNA polymerase: The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651–12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Polymerase Chain Reaction:

    Article Title: Efficient CRISPR/HDR-mediated knock-in system and method of use
    Article Snippet: .. The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651-12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Article Title: CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.
    Article Snippet: .. 58 | No. 4 | 2015 and the resulting clone was named pX330-LAsg. b) PCR amplification of DNA templates for synthesis of sgRNAs by in vitro transcription with T7 RNA polymerase: The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651–12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Amplification:

    Article Title: Efficient CRISPR/HDR-mediated knock-in system and method of use
    Article Snippet: .. The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651-12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Article Title: CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.
    Article Snippet: .. 58 | No. 4 | 2015 and the resulting clone was named pX330-LAsg. b) PCR amplification of DNA templates for synthesis of sgRNAs by in vitro transcription with T7 RNA polymerase: The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651–12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Plasmid Preparation:

    Article Title: Efficient CRISPR/HDR-mediated knock-in system and method of use
    Article Snippet: .. The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651-12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    Article Title: CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.
    Article Snippet: .. 58 | No. 4 | 2015 and the resulting clone was named pX330-LAsg. b) PCR amplification of DNA templates for synthesis of sgRNAs by in vitro transcription with T7 RNA polymerase: The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651–12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..

    In Vitro:

    Article Title: CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.
    Article Snippet: .. 58 | No. 4 | 2015 and the resulting clone was named pX330-LAsg. b) PCR amplification of DNA templates for synthesis of sgRNAs by in vitro transcription with T7 RNA polymerase: The template for the sgRNA (T3gRNA) targeting the T3 promoter sequence in the cloning vector A1 (pLACAGRFPTetOn; 21683 bp) was PCR amplified from pX330 using the forward primer T3gRNAF, which contained 3 parts: a T7 promoter sequence, the 19-bp guide sequence for the T3 promoter of vector A1 (position 12651–12669), and the first 20 bases of the tracrRNA sequence from the pX330 vector (16) (Addgene plasmid 42230) (Table 1). ..



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